RESPON MORFOGENESIS EKSPLAN PUCUK ANGGREK TEBU (Grammatophylum speciosum Blume) SECARA IN VITRO TERHADAP BEBERAPA KONSENTRASI KINETIN

Ellok Dwi Sulichantini, Susylowati Susylowati, Ariya Ramadhan

Abstract


Sugarcane orchid (Grammatophyllum speciosum Blume) are known as a type of giant orchid, adult orchids can weigh 1 ton, panicle can grow up to 2.5 – 3 meters with a diameter of about 1.5-2 cm. Each panicle has dozens, even reaching one hundred flower buds, each with a diameter of about one 10 cm. Sugarcane orchids are a type of plant that is protected because its existence is increasingly scarce so that it needs in vitro propagation techniques to support its survival.

To improve the growth of Grammatophyllum speciosum Blume in vitro shoot tip was growing on MS medium with varying concentration of kinetin (0, 1, 2, 3, and 4 mg L-1) with 5 replications. Shoot tip from in vitro culture around 1 cm lengt was used as explants. Observations carried out for 8 weeks after inoculation.

The results showed that shoot explants tip  growth well in MS basic medium. The addition of kinetin to the medium, increases growth rate of culture. Increasing the concentration of kinetin tends to increase the growth. The best concentration for growth induction on plant height, stem diameter,  leaves length and  length of roots was 2 mg.L-1 kinetin, On the other hand 3 mg.L-1 kinetin gave best response on number of leaves and 4  mg.L-1 kinetin was the best concentration on leaves width and number of roots.


Keywords


Grammatophyllum speciosum, Orchid, Kinetin, In Vitro

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References


Anonim. 1999. Jenis-jenis Tumbuhan dan Satwa yang Dilindungi. Lampiran Peraturan Pemerintah Republik Indonesia Nomor 7 Tahun 1999 Tanggal 27 Januari 1999.

Anonim, 2015. Pelestarian Grammatophyllum speciosum. Htttp://repisitori umy

Bojwani,S.S., and Radzan, 1983. Plant Tissue Culture: Theory and Practice. New York: Elsivier, Science Publishing Company.

CITES [Convention on International Trade in Endangered Species]. 2005.Checklist of CITES species. https: //cites.org/ eng/resources/pub/ checklist08/ Checklist.pdf

George, E.F., dan Sherrington. 1984. Plant Propagation by Tissue Culture. Handbook and Directory of Directory of Commercial laboratories. Eastern Press. England.

Gunawan, L.W. 1992. Teknik Kultur Jaringan Tumbuhan. Laboratorium Kultur Jaringan Tumbuhan Pusat Antar Universitas (PAU) Bioteknologi IPB, Bogor.

Hartmann, H.T., D.E. Kester, J.R. Davis, dan R.L. Geneve. 1997. Plant Propagation. Prentice-Hall International Inc. New Jersey.

Herawati, W. 2013. Konservasi Anggrek . Purwokerto: Universitas Jenderal Soedirman. http://bio.unsoed.ac.id/sites/default/files/Konservasi%20Anggrek-.pdf . DiaksesTanggal 19 Februari 2019.

Irawati. 2001. Tumbuhan langka Indonesia. Pusat Penelitian dan Pengembangan Biologi. LIPI. Balai Penelitian Botani Herbarium Bogoriense. Bogor.

Knudson, L. (1922) Nonsymbiotic Germination of Orchid Seeds. Botanical Gazette, 73, 1-25. https://doi.org/10.1086/332956 [Citation Time(s):12.

Kumaria, S. and Tandon, P. (2010) Biotechnological Approaches to Conservation of Orchids, the Wondrous and Mystic Plants of North-East India. Man and Society: A journal of North East Studies, 4, 61-68. [Citation Time(s):1]

Mondal, M., Gupta, S., Mukherjee, B.B. 1990. In Vitropropagation Of Shoot Buds of Carica papaya L. var. HoneyDew. Plant Cell Rep. 8:609-612.

O’Byrne, P. 1994. Lowland Orchids of Papua New Guinea. SNP Publishers, Singapore.

Pradhan, S., Tiruwa, B.L., Subedee, B.R. and Pant, B. (2016) Efficient Plant Regeneration of Cymbidium aloifolium (L.) Sw., a Threatened Orchid of Nepal through Artificial Seed Technology. American Journal of Plant Sciences, 7, 1964-1974. https://doi.org/10.4236/ajps.2016.714179

Sabran, M dan Firmansyah. 2015. Eksplorasi dan Karakterisasi Tanaman Anggrek di Kalimantan Tengah. Buletin PlasmaNutfah: Vol 19 No 1.

Salifah. H.A.B., Muskhazli, M., Rusea, G., Nithiyaa, P. 2011. Variation in mycorrhizal specificity for in vitro symbiotic seed germination of Grammatophyllum speciosum Blume. Sains Malaysiana 40 (5): 451- 455

Salisbury, F. dan Ross, C.W. 1995. Fisiologi Tumbuhan. Bandung. Penerbit ITB Bandung.

Santoso, U., dan F. Nursandi. 2004. Kultur Jaringan Tanaman.Universitas Muhammadiyah Malang Press. Malang.

Satyavathi, V.V., P.P. Jauhar, E.M. Elias, and M.B. Rao. 2004. Genomics, molecular genetic and biotechnology efects of growth regulators on in vitro plant regeneration. Crop Sci. (44): 1839-1846.

Wattimena, G.A. 1988. Zat Pengatur Tumbuh Tanaman. Laboratorium Kultur Jaringan, PAU Bioteknologi, Bogor.

Wiendi, N.M.A., G.A. Wattimena dan L.W. Gunawan. 1992. Perbanyakan Tanaman. 12-104. Dalam A.S. Abidin (ed.). Bioteknologi Tanaman. PAU, IPB. Bogor.

Wetherell, DF. 1982. Introduction to In Vitro Propagation Wayne. A very Publishing Group. New Jersey

Yukawa T, Kinoshita A, Tanaka N. 2013. Molecular identification resolves taxonomic confusion in Grammatophyllum speciosum complex (Orchidaceae). Bull Natl Museum Nat Sci 39 (3): 137-145.

Zulkarnain. 2009. Kultur Jaringan Tanaman: Solusi Perbanyakan Tanaman Budidaya. Bumi Aksara. Jakarta.




DOI: https://doi.org/10.31293/af.v19i2.4718

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